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pEASY?-Basic Seamless Cloning and Assembly Kit

基礎(chǔ)版同源重組無縫克隆試劑盒

目錄號: CU201-02

單 價:¥680

規(guī)格:
20 rxns
60 rxns
數(shù)量:
-
+
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產(chǎn)品詳情介紹

本產(chǎn)品利用特殊的重組酶和同源重組的原理,可以將任意方法線性化后的載體和與其兩端具有15-25 bp重疊區(qū)域

的PCR片段定向重組,可以實現(xiàn)1-5個片段的高效無縫拼接。

? 快速:僅需要5~15分鐘反應(yīng)時間。

? 簡單:不受片段酶切位點(diǎn)的影響,無需對片段酶切。

? 高效:陽性率達(dá)95% 以上。

? 無縫:不引入額外的序列。


產(chǎn)品組成

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實驗數(shù)據(jù)

組裝效率高


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References

1.You L, Omollo E O, Yu C, et al. Structural basis for intrinsic transcription termination[J]. Nature, 2023.(IF 64.80)

2.Huang J, Yang L, Yang L, et al. Stigma receptors control intraspecies and interspecies barriers in Brassicaceae[J]. Nature, 2023.(IF 64.80)

3.Liu R, Yang J, Yao J, et al. Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins[J]. Nature biotechnology, 2022.(IF 54.90)

4.Jiang L, Xie X, Su N, et al. Large Stokes shift fluorescent RNAs for dual-emission fluorescence and bioluminescence imaging in live cells[J]. Nature Methods, 2023.(IF 48.00)

5.Wu M, Xu G, Han C, et al. lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription[J]. Science, 2021.(IF 47.73)

6.Zhang R, He Z, Shi Y, et al. Amplification editing enables efficient and precise duplication of DNA from short sequence to megabase and chromosomal scale[J]. Cell, 2024.(IF 45.50)

7.Bai X, Sun P, Wang X, et al. Structure and dynamics of the EGFR/HER2 heterodimer[J]. Cell Discovery, 2023.(IF 33.50)

8.Lei X, Huang A, Chen D, et al. Rapid generation of long, chemically modified pegRNAs for prime editing[J]. Nature Biotechnology, 2024.(IF 33.10)

9.Zou Z P, Du Y, Fang T T, et al. Biomarker-responsive engineered probiotic diagnoses, records, and ameliorates inflammatory bowel disease in mice[J]. Cell Host & Microbe, 2022.(IF 31.32)

10.Zhang Y, Dai F, Chen N, et al. Structural insights into VAChT neurotransmitter recognition and inhibition[J]. Cell Research, 2024.(IF 28.10)

11.Yan X, Li Y, Wang G, et al. Molecular basis for recognition of Gly/N-degrons by CRL2ZYG11B and CRL2ZER1[J]. Molecular Cell, 2021.(IF 17.97)

12.Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022,(IF 17.35)

13.Jiang C, Yu C, Sun S, et al. A new anti-CRISPR gene promotes the spread of drug-resistance plasmids in Klebsiella pneumoniae[J]. Nucleic Acids Research, 2024.(IF 16.60)

14.Wan W, Dong H, Lai D H, et al. The Toxoplasma micropore mediates endocytosis for selective nutrient salvage from host cell compartments[J]. Nature communications, 2023.(IF 16.60)

15.Ye D, Shao Y, Li W R, et al. Characterization and Engineering of Two Highly Paralogous Sesquiterpene Synthases Reveal a Regioselective Reprotonation Switch[J]. Angewandte Chemie International Edition, 2024.(IF 16.60)

16.Zhang H W, Huang K, Gu Z X, et al. A cryo-EM structure of KTF1-bound polymerase V transcription elongation complex[J]. Nature Communications, 2023.(IF 16.60)

17.Wang T, Zou J, Wang K, et al. Chemoenzymatic Synthesis of the Cyclopiane Family of Diterpenoid Natural Products[J]. Angewandte Chemie International Edition, 2025(IF 16.10)

18.Chen Q, Hu T, Li X, et al. Phosphorylation of SWEET sucrose transporters regulates plant root: shoot ratio under drought[J]. Nature plants, 2022.(IF 15.79)

19.Chen D, Song Z, Han J, et al. Targeted Discovery of Glycosylated Natural Products by Tailoring Enzyme-Guided Genome Mining and MS-Based Metabolome Analysis[J]. Journal of the American Chemical Society, 2024.(IF 15.00)

20.Wan H, Zhong X, Yang S, et al. Enhancing the Therapeutic Potential of Peptide Antibiotics Using Bacteriophage Mimicry Strategies[J]. Advanced Science, 2025.(IF 14.10)

21.Nian X, Wang B, Holford P, et al. Neuropeptide Ecdysis‐Triggering Hormone and Its Receptor Mediate the Fecundity Improvement of ‘Candidatus Liberibacter Asiaticus’‐Infected Diaphorina citri Females and CLas Proliferation[J]. Advanced Science, 2025.(IF 14.10)

22.Liu R, Yao J, Zhou S, et al. Spatiotemporal control of RNA metabolism and CRISPR–Cas functions using engineered photoswitchable RNA-binding proteins[J]. Nature Protocols, 2023.(IF 13.10)

23.Zuo F, Jiang L, Su N, et al. Imaging the dynamics of messenger RNA with a bright and stable green fluorescent RNA[J]. Nature Chemical Biology, 2024.(IF 13.00)

24.Fang M, Li H, Xie X, et al. Imaging intracellular metabolite and protein changes in live mammalian cells with bright fluorescent RNA-based genetically encoded sensors[J]. Biosensors and Bioelectronics, 2023.(IF 12.60)

25.Shi Z, Liu G, Jiang H, et al. Activation of P53 pathway contributes to Xenopus hybrid inviability[J]. Proceedings of the National Academy of Sciences, 2023.(IF 11.10)

26.Zhang L, Huang J, Su S, et al. FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa[J]. Current Biology, 2021.(IF 10.83)

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