TransDetect? Cell Counting Kit (CCK)
細(xì)胞增殖及細(xì)胞毒性檢測試劑盒
目錄號(hào)： FC101-01
單 價(jià)：￥220
產(chǎn)品詳情介紹
TransDetect? Cell Counting Kit，是一種基于水溶性四唑鹽的細(xì)胞增殖和細(xì)胞毒性檢測試劑盒。它在電子耦合試劑1-Methoxy PMS存在的情況下，可以被線粒體內(nèi)的脫氫酶還原為可溶性的橙黃色甲臜(formazan)。甲臜的數(shù)量與活細(xì)胞的數(shù)量成正比。細(xì)胞增殖越快、細(xì)胞毒性越小、細(xì)胞數(shù)量越多，則顏色越深，顏色的深淺與細(xì)胞數(shù)量呈現(xiàn)良好的線性關(guān)系。該產(chǎn)品細(xì)胞毒性小，對后續(xù)實(shí)驗(yàn)沒有影響，與MTT，XTT，MTS和WST-1相比，此法檢測靈敏度更高，線性范圍更寬，適用于藥物篩選，細(xì)胞增殖測定，細(xì)胞毒性測定和腫瘤藥敏實(shí)驗(yàn)。
CCK靈敏度檢測
CCK對細(xì)胞毒性低
1.Zhao M M, Zhu Y, Zhang L, et al. Novel cleavage sites identified in SARS-CoV-2 spike protein reveal mechanism for cathepsin L-facilitated viral infection and treatment strategies[J]. Cell Discovery, 2022.(IF 38.07)
2.Liu Q, Huang Y, Li L, et al. Endogenous Enzyme‐Operated Spherical Nucleic Acids for Cell‐Selective Protein Capture and Localization Regulation[J]. Angewandte Chemie International Edition, 2023.(IF 16.60)
3.Liao N, Su L, Cao Y, et al. Tracking cell viability for adipose-derived mesenchymal stem cell-based therapy by quantitative fluorescence imaging in the second near-infrared window[J]. ACS nano, 2022.(IF 15.88)
4.Liao N, Su L, Zheng Y, et al. In vivo tracking of cell viability for adoptive natural killer cell‐based immunotherapy by ratiometric NIR‐II fluorescence imaging[J]. Angewandte Chemie, 2021.(IF 15.34)
5.Zhao J, Chu H, Zhao Y, et al. A NIR light gated DNA nanodevice for spatiotemporally controlled imaging of microRNA in cells and animals[J]. Journal of the American Chemical Society, 2019.(IF 14.69)
6.Cai B, Li Z, Ma M, et al. Long noncoding RNA SMUL suppresses SMURF2 production-mediated muscle atrophy via nonsense-mediated mRNA decay[J]. Molecular Therapy Nucleic Acids, 2021(IF 11.45)
7.Cai B, Ma M, Zhang J, et al. LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2[J]. Molecular Therapy Nucleic Acids, 2022.(IF 8.89)
8.Cai B, Ma M, Zhang J, et al. Long noncoding RNA ZFP36L2-AS functions as a metabolic modulator to regulate muscle development[J]. Cell Death & Disease, 2022.(IF 8.46)
9.Ma M, Cai B, Zhou Z, et al. LncRNA-TBP mediates TATA-binding protein recruitment to regulate myogenesis and induce slow-twitch myofibers[J]. Cell Communication and Signaling, 2023.(IF 7.52)
10.Zhong F, Liu J, Gao C, et al. Downstream regulatory network of MYBL2 mediating its oncogenic role in melanoma[J]. Frontiers in Oncology, 2022.(IF 6.24)
11.Cai B, Ma M, Chen B, et al. MiR-16-5p targets SESN1 to regulate the p53 signaling pathway, affecting myoblast proliferation and apoptosis, and is involved in myoblast differentiation[J]. Cell death & disease, 2018,(IF 5.64)
12.Yu J, Wang Z, Yang X, et al. LncRNA-FKBP1C regulates muscle fiber type switching by affecting the stability of MYH1B[J]. Cell Death Discovery, 2021.(IF 5.24)
13.Cai B, Ma M, Zhou Z, et al. circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis[J]. Journal of Animal Science and Biotechnology, 2022.(IF 5.03)
