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ProteinIso? Ni-NTA Resin

Ni-NTA Resin  蛋白純化

目錄號(hào): DP101-01

單 價(jià):¥570

規(guī)格:
5 ml
25 ml
數(shù)量:
-
+
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產(chǎn)品詳情介紹

ProteinIso? Ni-NTA Resin是螯合金屬Ni2+而形成的一種親和層析介質(zhì)。NTA能夠通過(guò)四個(gè)位點(diǎn)牢固地螯合Ni2+,從而減少純化過(guò)程中Ni2+泄漏到蛋白樣品中。本產(chǎn)品對(duì)His標(biāo)簽蛋白具有特異吸附能力,從而使His標(biāo)簽蛋白結(jié)合在Ni-NTA純化介質(zhì)上,未結(jié)合的蛋白被洗滌下去。結(jié)合在介質(zhì)上的蛋白經(jīng)過(guò)一定濃度的咪唑或者pH低緩沖液溫和洗脫下來(lái),從而得到高純度的目的蛋白。本產(chǎn)品具有吸附容量大、選擇性好、通透性強(qiáng)、易于再生等優(yōu)點(diǎn),適用于在非變性或變性條件下純化任何表達(dá)系統(tǒng)表達(dá)的His標(biāo)簽重組蛋白。

實(shí)驗(yàn)數(shù)據(jù)

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References

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2.Guo L, Zhao Y, Zhang Q, et al. Stochastically multimerized ParB orchestrates DNA assembly as unveiled by single-molecule analysis[J]. Nucleic Acids Research, 2022.(IF 19.16)

3.Zhou C J, Wang X Y, Dong Y H, et al. CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I[J]. Nature communications, 2022,(IF 17.69)

4.Zhang Q, Chen Z, Wang F, et al. Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer[J]. Nucleic Acids Research, 2021.(IF 16.97)

5.Zhou B, Luo Q, Shen Y, et al. Coordinated regulation of vegetative phase change by brassinosteroids and the age pathway in Arabidopsis[J]. Nature Communications, 2023.(IF 16.60)

6.Wu W K, Nie G B, Lin J L, et al. Regulation of glandular size and phytoalexin biosynthesis by a negative feedback loop in cotton[J]. Advanced Science, 2024,(IF 14.30)

7.Zhang Y, Gao Y, Wang H L, et al. Verticillium dahliae secretory effector PevD1 induces leaf senescence by promoting ORE1-mediated ethylene biosynthesis[J]. Molecular plant, 2021.(IF 13.16)

8.Wei Y, Liu Z, Lv T, et al. Ethylene enhances MdMAPK3-mediated phosphorylation of MdNAC72 to promote apple fruit softening[J]. The Plant Cell, 2023.(IF 11.60)

9.Zheng W, Li S, Shi Z, et al. Recombinant ferritin-based nanoparticles as neoantigen carriers significantly inhibit tumor growth and metastasis[J]. Journal of Nanobiotechnology, 2024.(IF 10.60)

10.Liu Y, Yu T F, Li Y T, et al. Mitogen‐activated protein kinase TaMPK3 suppresses ABA response by destabilising TaPYL4 receptor in wheat[J]. New Phytologist, 2022.(IF 10.32)

11.Hu C, Wu S, Li J, et al. Herbivore‐induced Ca2+ signals trigger a jasmonate burst by activating ERF16‐mediated expression in tomato[J]. New Phytologist, 2022.(IF 10.32)

12.Gao Y, Li Z, Yang C, et al. Pseudomonas syringae activates ZAT18 to inhibit salicylic acid accumulation by repressing EDS1 transcription for bacterial infection[J]. New Phytologist, 2022.(IF 10.15)

13.Gao J, Zhao B R, Zhang H, et al. Interferon functional analog activates antiviral Jak/Stat signaling through integrin in an arthropod[J]. Cell reports, 2021,(IF 9.42)

14.Li P, Tan X, Liu R, et al. QTL detection and candidate gene analysis of grape white rot resistance by interspecific grape (Vitis vinifera L.× Vitis davidii Foex.) crossing[J]. Horticulture Research, 2023.(IF 8.70)

15.Chen K, Sun W, Zhong M, et al. Single-molecule assay guided crRNA optimization enhances specific microRNA detection by CRISPR-Cas12a[J]. Sensors and Actuators B: Chemical, 2024.(IF 8.40)

16.Fu W, Liu Y, Liu F, et al. A novel autoantibody induced by bacterial biofilm conserved components aggravates lupus nephritis[J]. Frontiers in Immunology, 2021.(IF 7.56)

17.Xu Y, Liu Z, Lv T, et al. Exogenous Ca2+ promotes transcription factor phosphorylation to suppress ethylene biosynthesis in apple[J]. Plant Physiology, 2023.(IF 7.40)

18.Song M, Fan X, Chen J, et al. OsNAR2. 1 interaction with OsNIT1 and OsNIT2 functions in root-growth responses to nitrate and ammonium[J]. Plant physiology, 2020,(IF 6.90)

19.Wang F P, Zhao P P, Zhang L, et al. The VvWRKY37 Regulates Bud Break in Grape Vine Through ABA-Mediated Signaling Pathways[J]. Frontiers in Plant Science, 2022.(IF 6.62)

20.Wang Z, Wang S, Xu Z, et al. Highly promiscuous flavonoid 3-O-glycosyltransferase from Scutellaria baicalensis[J]. Organic letters, 2019.(IF 6.55)

21.Xiao M, Wang B, Feng Y, et al. Three candidate 2-(2-phenylethyl) chromone-producing type III polyketide synthases from Aquilaria sinensis (Lour.) Gilg have multifunctions synthesizing benzalacetones, quinolones and pyrones[J]. Industrial Crops and Products, 2022.(IF 6.45)

22.Li X J, Yang L, Li D, et al. Pathogen-specific binding soluble down syndrome cell adhesion molecule (Dscam) regulates phagocytosis via membrane-bound Dscam in crab[J]. Frontiers in immunology, 2018,(IF 6.43)

23.Liu C, Shen N, Zhang Q, et al. Magnaporthe oryzae transcription factor MoBZIP3 regulates appressorium turgor pressure formation during pathogenesis[J]. International Journal of Molecular Sciences, 2022.(IF 6.21)

24.Liu S, Zhang M, Bao Y, et al. Characterization of a Highly Selective 2 ″-O-Galactosyltransferase from Trollius chinensis and structure-guided engineering for improving UDP-glucose selectivity[J]. Organic Letters, 2021.(IF 6.00)

25.Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57)

26.Liu X, Zhu X, Wei X, et al. The wheat LLM-domain-containing transcription factor TaGATA1 positively modulates host immune response to Rhizoctonia cerealis[J]. Journal of Experimental Botany, 2020.(IF 5.36)

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